■ 基本信息
別名: | pFastBacI |
啟動(dòng)子: | PH |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 廣宿主系列，昆蟲(chóng)細(xì)胞載體 |
質(zhì)粒大小: | 4776bp |
原核抗性: | Amp |
篩選標(biāo)記: | 慶大霉素Gen |
克隆菌株: | DH5α |
培養(yǎng)條件: | 37℃ |
表達(dá)宿主: | 昆蟲(chóng)細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | pFastbac-F:TATTCCGGATTATTCATACC |
3'測(cè)序引物: | pFastBac-R:ACAAATGTGGTATGGCTGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲(chóng)細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp,Gen |
真核抗性: |
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pFastBac1載體是非融合性蛋白表達(dá)載體，在載體內(nèi)部不帶有其他融合性的蛋白標(biāo)簽。為了保證目的基因的正確表達(dá)，插入片段必須包含ATG起始密碼子，保證蛋白起始翻譯。插入片段最好包含終止密碼子，保證蛋白質(zhì)不會(huì)翻譯出多余的氨基酸。在pFastBacI載體多克隆位點(diǎn)中，對(duì)于所有的三種讀碼框分布，都包含有終止密碼子。
The Bac-to-Bac? Vector Kit contains a pFastBac? 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac? Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac? System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:
Time-saving expression bacmid. With Bac-to-Bac?, the expression cassette of the pFastBac? vector recombines with the parent bacmid in DH10Bac? E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
Easy colony screening. The parent bacmid in DH10Bac? E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1037pFastBac1昆蟲(chóng)胞內(nèi)質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
