■ 基本信息
啟動(dòng)子: | 無，需要克隆進(jìn)啟動(dòng)子才能表達(dá) |
復(fù)制子: | pVS1V |
終止子: | 無 |
質(zhì)粒大小: | 11849bp |
原核抗性: | Kan |
真核抗性: | Hyg,Gus |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物細(xì)胞 |
5'測(cè)序引物: | M13F |
3'測(cè)序引物: | M13R |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | Hyg
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use these vectors to create fusions of gusA with their gene of interest. These vectors contain the pUC18 polylinker-lacZa.
本質(zhì)粒的參考文獻(xiàn)：A specific allele of MYB14 in grapevine correlates with high stilbene inducibility triggered by Al3+ and UV-C radiation.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK858pCAMBIA1301植物表達(dá)質(zhì)粒.txt
