■ 基本信息
別名: | TRC2-pLKO-Puro;pLKO.5-TRC2-Puro |
啟動子: | U6 |
復制子: | pUC |
質粒分類: | 病毒系列，慢病毒干擾載體 |
質粒大小: | 7484bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物:
| U6:ATGGACTATCATATGCTTACCGTA |
3'測序引物: | 根據序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 基因沉默 |
片段類型: | shRNA
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: | Puro
|
熒光標記: |
|
■ 質粒簡介
The MISSION TRC2 Control Vector pLKO-puro is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones.
