■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | hGH poly(A) signal |
質(zhì)粒分類(lèi): | 病毒系列，腺病毒相關(guān)克隆載體 |
質(zhì)粒大小: | 5904bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測(cè)序引物: | hGH-PA-R: CCAGCTTGGTTCCCAATAGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,腺相關(guān)病毒 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: | 綠色
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■ 質(zhì)粒簡(jiǎn)介
The pAAV-ZsGreen1 Vector is an adeno-associated virus (AAV) vector that contains the ZsGreen1 gene. This vector can be used as a positive control to monitor AAV particle production using AAVpro Helper Free Systems. Expression of ZsGreen, a variant of the green fluorescent protein from Zoanthus sp., can be used to assess transfection and transduction efficiency during AAV particle preparation.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK732pAAV-IRES-ZsGreen1腺相關(guān)質(zhì)粒.txt
